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Novus Biologicals city state drp1 rabbit polyclonal

Interaction of designed drug molecules against Aβ and <t>Drp1</t> Complex. (A) Interaction of Drp1 (PDB ID: 4h1u) and Aβ1–40 (PDBID: 1ba4) proteins. (B) Inhibition of Drp1 and Aβ40 peptide Interaction by DDQ. (C) Interaction of Drp1 and designed drug molecules. We designed 82 molecules based on existing mitochondrial division inhibiting drug molecules in AD and subjected to molecular docking studies. We prepared Aβ and Drp1 complex and introduced these molecular structures into this complex. Few of these molecule showed better docking score compared to existed drug molecules. Amongst DDQ is only involved in the prevention of Aβ-Drp1 interaction by interacting at the active site such as ser8 and Leu34 of Aβ and ASN16 Glu16 of Drp1. And also DDQ showed better binding score (-10.8462) than existed drug molecules. DDQ is readily stopping the Drp1 before forming the complex. DDQ exhibited binding interaction at Arg225 (C = O→O-P) and phenyl part of DDQ is also showing one arene cationic interaction at Arg225. Hence, among all designed molecules we selected only DDQ for our further steps.

City State Drp1 Rabbit Polyclonal, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 44 article reviews

city state drp1 rabbit polyclonal - by Bioz Stars, 2026-10

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1) Product Images from "Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities"

Journal: Human Molecular Genetics

doi: 10.1093/hmg/ddx226

Interaction of designed drug molecules against Aβ and Drp1 Complex. (A) Interaction of Drp1 (PDB ID: 4h1u) and Aβ1–40 (PDBID: 1ba4) proteins. (B) Inhibition of Drp1 and Aβ40 peptide Interaction by DDQ. (C) Interaction of Drp1 and designed drug molecules. We designed 82 molecules based on existing mitochondrial division inhibiting drug molecules in AD and subjected to molecular docking studies. We prepared Aβ and Drp1 complex and introduced these molecular structures into this complex. Few of these molecule showed better docking score compared to existed drug molecules. Amongst DDQ is only involved in the prevention of Aβ-Drp1 interaction by interacting at the active site such as ser8 and Leu34 of Aβ and ASN16 Glu16 of Drp1. And also DDQ showed better binding score (-10.8462) than existed drug molecules. DDQ is readily stopping the Drp1 before forming the complex. DDQ exhibited binding interaction at Arg225 (C = O→O-P) and phenyl part of DDQ is also showing one arene cationic interaction at Arg225. Hence, among all designed molecules we selected only DDQ for our further steps.

Figure Legend Snippet: Interaction of designed drug molecules against Aβ and Drp1 Complex. (A) Interaction of Drp1 (PDB ID: 4h1u) and Aβ1–40 (PDBID: 1ba4) proteins. (B) Inhibition of Drp1 and Aβ40 peptide Interaction by DDQ. (C) Interaction of Drp1 and designed drug molecules. We designed 82 molecules based on existing mitochondrial division inhibiting drug molecules in AD and subjected to molecular docking studies. We prepared Aβ and Drp1 complex and introduced these molecular structures into this complex. Few of these molecule showed better docking score compared to existed drug molecules. Amongst DDQ is only involved in the prevention of Aβ-Drp1 interaction by interacting at the active site such as ser8 and Leu34 of Aβ and ASN16 Glu16 of Drp1. And also DDQ showed better binding score (-10.8462) than existed drug molecules. DDQ is readily stopping the Drp1 before forming the complex. DDQ exhibited binding interaction at Arg225 (C = O→O-P) and phenyl part of DDQ is also showing one arene cationic interaction at Arg225. Hence, among all designed molecules we selected only DDQ for our further steps.

Techniques Used: Inhibition, Binding Assay

mRNA fold changes of mitochondrial structural, mitochondrial biogenesis and synaptic genes in Human Neuroblastoma (SHSY5Y) cells treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ relative to the untreated SHSY5Y cells and cells treated with Aβ+DDQ and DDQ + Aβ relative to the Aβ-treated SHSY5Y cells

Figure Legend Snippet: mRNA fold changes of mitochondrial structural, mitochondrial biogenesis and synaptic genes in Human Neuroblastoma (SHSY5Y) cells treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ relative to the untreated SHSY5Y cells and cells treated with Aβ+DDQ and DDQ + Aβ relative to the Aβ-treated SHSY5Y cells

Techniques Used:

Co-immunoprecipitation analysis of Drp1 and Aβ in SHSY5Y cells. (A) represents immunoprecipitation with the 6E10 antibody and immunoblotting with the 6E10 antibody, indicating that the specificity of 6E10 in our Co-IP analysis. In cells treated with DDQ + Aβ and Aβ+DDQ, 4 kDa Aβ levels were reduced relative cells treated Aβ alone. (B) represents Co-IP with Aβ antibody 6E10 and western blotting with Drp1 antibody, indicating that Drp1 interacts with 4 kDa Aβ. Reduced interaction between Aβ and Drp1 was found in cells pretreated with DDQ and then Aβ added. Reduced interaction was strong in DDQ + Aβ cells compared to cells treated with Aβ alone.

Figure Legend Snippet: Co-immunoprecipitation analysis of Drp1 and Aβ in SHSY5Y cells. (A) represents immunoprecipitation with the 6E10 antibody and immunoblotting with the 6E10 antibody, indicating that the specificity of 6E10 in our Co-IP analysis. In cells treated with DDQ + Aβ and Aβ+DDQ, 4 kDa Aβ levels were reduced relative cells treated Aβ alone. (B) represents Co-IP with Aβ antibody 6E10 and western blotting with Drp1 antibody, indicating that Drp1 interacts with 4 kDa Aβ. Reduced interaction between Aβ and Drp1 was found in cells pretreated with DDQ and then Aβ added. Reduced interaction was strong in DDQ + Aβ cells compared to cells treated with Aβ alone.

Techniques Used: Immunoprecipitation, Western Blot, Co-Immunoprecipitation Assay

Co-immunoprecipitation analysis of mutant APPSwe/Ind cells treated with DDQ. We transfected mutant APPSwe cDNA construct into mouse neuroblastoma (N2a) cells. After 24 h of transfection, cells were treated with DDQ (250nM) for 24 h. Harvested mutant APPSwe/Ind cells treated and untreated with DDQ and prepared protein lysates and performed immunoprecipitation with Aβ (6E10) antibody and conducted immunoblotting analysis with 6E10 and Drp1 antibodies. Lanes 1 and 2 represents IP with 6E10 and western blot with 6E10 and lanes 3 and 4 represents IP with 6E10 and western blot with Drp1 antibody respectively. As shown in Figure, reduced levels of full-length APP and 4 kDa Aβ were found in lane 2 mutant APPSwe/Ind cells treated with DDQ compared to lane 1 of mutant APPSwe/Ind cells untreated with DDQ. Reduced levels of Drp1 were found in lane 4 of mutant APPSwe cells treated with DDQ compared to lane 3 of mutant APPSwe/Ind cells untreated with DDQ.

Figure Legend Snippet: Co-immunoprecipitation analysis of mutant APPSwe/Ind cells treated with DDQ. We transfected mutant APPSwe cDNA construct into mouse neuroblastoma (N2a) cells. After 24 h of transfection, cells were treated with DDQ (250nM) for 24 h. Harvested mutant APPSwe/Ind cells treated and untreated with DDQ and prepared protein lysates and performed immunoprecipitation with Aβ (6E10) antibody and conducted immunoblotting analysis with 6E10 and Drp1 antibodies. Lanes 1 and 2 represents IP with 6E10 and western blot with 6E10 and lanes 3 and 4 represents IP with 6E10 and western blot with Drp1 antibody respectively. As shown in Figure, reduced levels of full-length APP and 4 kDa Aβ were found in lane 2 mutant APPSwe/Ind cells treated with DDQ compared to lane 1 of mutant APPSwe/Ind cells untreated with DDQ. Reduced levels of Drp1 were found in lane 4 of mutant APPSwe cells treated with DDQ compared to lane 3 of mutant APPSwe/Ind cells untreated with DDQ.

Techniques Used: Immunoprecipitation, Mutagenesis, Transfection, Construct, Western Blot

Double labeling Immunofluorescence analysis of Drp1 and Aβ. Double-labeling immunofluorescence analysis of Aβ (6E10 antibody) and Drp1 in SHSY5Y cells. The localization of Drp1 (green) and Aβ (red) and the colocalization of Drp1 and Aβ (yellow, merged) at 60× the original magnification. Top panel, represents Aβ-treated cells, middle panel shows Aβ+DDQ-treated cells and the bottom panel shows DDQ+Aβ-treated cells. As shown in Figure 7, increased levels of Drp1 and intra-neuronal Aβ (full-length APP) and colocalization of Drp1 and Aβ in top panel, where as in the middle panel Aβ+DDQ cells, reduced Drp1 and Aβ and also reduced colocalization and in the bottom panel Drp1 and Aβ levels markedly reduced compared to top panel and also colocalization of Drp1 and Aβ. These findings strongly suggest that DDQ 1) reduces Drp1 and Aβ levels and also 2) inhibit the interaction of Drp1 and Aβ in SHSY5Y cells. These findings agree with our Co-IP findings.

Figure Legend Snippet: Double labeling Immunofluorescence analysis of Drp1 and Aβ. Double-labeling immunofluorescence analysis of Aβ (6E10 antibody) and Drp1 in SHSY5Y cells. The localization of Drp1 (green) and Aβ (red) and the colocalization of Drp1 and Aβ (yellow, merged) at 60× the original magnification. Top panel, represents Aβ-treated cells, middle panel shows Aβ+DDQ-treated cells and the bottom panel shows DDQ+Aβ-treated cells. As shown in Figure 7, increased levels of Drp1 and intra-neuronal Aβ (full-length APP) and colocalization of Drp1 and Aβ in top panel, where as in the middle panel Aβ+DDQ cells, reduced Drp1 and Aβ and also reduced colocalization and in the bottom panel Drp1 and Aβ levels markedly reduced compared to top panel and also colocalization of Drp1 and Aβ. These findings strongly suggest that DDQ 1) reduces Drp1 and Aβ levels and also 2) inhibit the interaction of Drp1 and Aβ in SHSY5Y cells. These findings agree with our Co-IP findings.

Techniques Used: Labeling, Immunofluorescence, Co-Immunoprecipitation Assay

Mitochondrial function. Mitochondrial functional parameters in control human neuroblastoma (SHSY5Y) cells, in amyloid β (Aβ) incubated SHSY5Y cells, in SHSY5Y cells treated with DDQ and in SHSY5Y cells incubated with Aβ and then treated with DDQ and in SHSY5Y cells treated with DDQ and then incubated with Aβ (n = 4). We analyzed mitochondrial functional data in two ways: (1) the control SHSY5Y cells were compared with the SHSY5Y cells treated with Aβ, DDQ, Aβ+DDQ and DDQ + Aβ and (2) Aβ-incubated SHSY5Y cells were compared with Aβ+DDQ SHSY5Y cells and DDQ + Aβ-treated cells. We performed statistical analysis using ANOVA following the Dunnett correction, for: (a) H2O2 production, (b) lipid peroxidation, (c) cytochrome oxidase activity, (d) ATP levels, and (e) GTPase-Drp1 activity.

Figure Legend Snippet: Mitochondrial function. Mitochondrial functional parameters in control human neuroblastoma (SHSY5Y) cells, in amyloid β (Aβ) incubated SHSY5Y cells, in SHSY5Y cells treated with DDQ and in SHSY5Y cells incubated with Aβ and then treated with DDQ and in SHSY5Y cells treated with DDQ and then incubated with Aβ (n = 4). We analyzed mitochondrial functional data in two ways: (1) the control SHSY5Y cells were compared with the SHSY5Y cells treated with Aβ, DDQ, Aβ+DDQ and DDQ + Aβ and (2) Aβ-incubated SHSY5Y cells were compared with Aβ+DDQ SHSY5Y cells and DDQ + Aβ-treated cells. We performed statistical analysis using ANOVA following the Dunnett correction, for: (a) H2O2 production, (b) lipid peroxidation, (c) cytochrome oxidase activity, (d) ATP levels, and (e) GTPase-Drp1 activity.

Techniques Used: Functional Assay, Control, Incubation, Activity Assay

Summary of real-time RT-PCR oligonucleotide primers used in measuring mRNA expression in mitochondrial and synaptic genes in untreated-SHSY5Y, DDQ-SHSY5Y, Aβ-SHSY5Y, Aβ+DDQ-SHSY5Y and DDQ + Aβ-SHSY5Y treated cell line

Figure Legend Snippet: Summary of real-time RT-PCR oligonucleotide primers used in measuring mRNA expression in mitochondrial and synaptic genes in untreated-SHSY5Y, DDQ-SHSY5Y, Aβ-SHSY5Y, Aβ+DDQ-SHSY5Y and DDQ + Aβ-SHSY5Y treated cell line

Techniques Used: Quantitative RT-PCR, Expressing, Sequencing

Summary of antibody dilutions and conditions used in the immunoblotting analysis of mitochondrial structural and synaptic proteins in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ

Figure Legend Snippet: Summary of antibody dilutions and conditions used in the immunoblotting analysis of mitochondrial structural and synaptic proteins in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ

Techniques Used: Western Blot

Figure Legend Snippet: Summary of antibody dilutions and conditions used in the co-immunoprecipitation

Techniques Used:

Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ

Figure Legend Snippet: Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ

Techniques Used: Immunohistochemistry

Figure Legend Snippet: Molecular docking of the Drp1 compounds

Techniques Used:

Marker:

Article Title: Mitochondria-targeted small molecule SS31: a potential candidate for the treatment of Alzheimer’s disease
Article Snippet: .. Statistical significance was assessed, using a one-way ANOVA for mitochondrial and synaptic and mitochondrial proteins. table ft1 table-wrap mode="anchored" t5 caption a7 Marker Primary antibody—species and dilution Purchased from company, city & state Secondary antibody, dilution Alexa Fluor dye Purchased from company, city & state Drp1 Rabbit polyclonal 1:300 Novus Biological, Littleton, CO Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA Mfn1 Rabbit polyclonal 1:300 Protein Tech Group, Inc., Chicago, IL Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA Mfn2 Rabbit polyclonal 1:200 Protein Tech Group, Inc., Chicago, IL Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA PGC1α Rabbit polyclonal 1:300 Novus Biological, Littleton, CO Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA Nrf1 Rabbit polyclonal 1:200 Santa Cruz Biotechnology, Inc., Dallas, TX Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA Nrf2 Rabbit polyclonal 1:300 Novus Biological, Littleton, CO Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA TFAM Rabbit polyclonal 1:300 Novus Biological, Littleton, CO Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA SYN Rabbit polyclonal 1:400 Protein Tech Group, Inc., Chicago, IL Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA PSD95 Rabbit polyclonal 1:400 Protein Tech Group, Inc., Chicago, IL Donkey Anti-rabbit IgG Alexa Fluor 488 Conjugate Thermo Fisher Scientific, Waltham, MA 6E10 Mouse monoclonal 1:400 Biolegend, San Diego, CA Donkey Anti-mouse IgG Alexa Fluor 594 Conjugate Thermo Fisher Scientific, Waltham, MA Open in a separate window Summary of antibody dilutions and conditions used in the immunofluorescence analysis of mitochondrial dynamics, mitochondrial biogenesis, and synaptic proteins in 12-month-old SS31-treated and untreated APP mice Measurement of soluble Aβ levels The cerebral cortex of each APP mouse brain was snap-frozen on dry ice at the time of sacrifice and stored at −70 °C until a homogenate was prepared. ..

Article Title: Mitochondria-targeted small molecule SS31: a potential candidate for the treatment of Alzheimer’s disease
Article Snippet: .. An ID fine-band command was used to locate and to scan the bands in each lane and to record the readings. table ft1 table-wrap mode="anchored" t5 caption a7 Marker Primary antibody – species and dilution Purchased from company, city & state Secondary antibody, dilution Purchased from company, city & state Drp1 Rabbit polyclonal 1:500 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ Mfn1 Rabbit polyclonal 1:400 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ Mfn2 Rabbit polyclonal 1:400 Abcam, Cambridge, MA Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ SYN Rabbit monoclonal 1:400 Abcam, Cambridge, MA Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ PSD95 Rabbit monoclonal 1:300 Abcam, Cambridge, MA Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ PGC1α Rabbit polyclonal 1:500 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ Nrf1 Rabbit polyclonal 1:300 Santa Cruz Biotechnology, Inc., Dallas, TX Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ Nrf2 Rabbit polyclonal 1:300 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ TFAM Rabbit polyclonal 1:300 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ 6E10 Mouse monoclonal 1:500 BioLegend San Diego, CA Sheep Anti-mouse HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ B-actin Mouse monoclonal 1:500 Sigma–Aldrich, St Luis, MO Sheep Anti-mouse HRP 1:10 000 GE Healthcare Amersham, Piscataway, NJ Open in a separate window Summary of antibody dilutions and conditions used in immunoblotting analysis of mitochondrial dynamics, mitochondrial biogenesis, and synaptic proteins in 12-month-old SS31-treated and untreated APP mice Immunofluorescence analysis and quantification Immunofluorescence analysis was performed using midbrain sections from the SS31-treated APP mice and the untreated APP mice, as described in Manczak and Reddy ( 67 ). ..

Article Title: Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities
Article Snippet: .. Details of proteins, dilutions of antibodies used for immunoblotting analysis was given in . table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Marker Primary antibody – species and dilution Purchased from Company, State Secondary antibody, dilution Purchased from Company, City & State Drp1 Rabbit Polyclonal 1:500 Novus Biological, Littleton, CO Donkey anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ Fis1 Rabbit Polyclonal 1:500 MBL International Corporation Woburn, Ma Donkey anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ Mfn1 Rabbit Polyclonal 1:400 Novus Biological, Littleton, CO Donkey anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ - Mfn2 Rabbit Polyclonal 1:400 Novus Biological, Littleton, CO Donkey anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ SYN Rabbit Monoclonal 1:400 Rabbit Monoclonal 1:400 Donkey anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ PSD95 Rabbit Monoclonal 1:300 Abcam, Cambridge, MA Donkey anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ PGC1α Rabbit Polyclonal 1:500 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ Nrf1 Mouse Monoclonal 1:30 Abcam, Cambridge, MA Sheep anti-mouse HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ Nrf2 Rabbit Polyclonal 1:300 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ TFAM Rabbit Polyclonal 1:30 Novus Biological, Littleton, CO Donkey Anti-rabbit HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ B-actin Mouse Monoclonal 1:500 Sigma-Aldrich, St Luis, MO Sheep anti-mouse HRP 1:10,000 GE Healthcare Amersham, Piscataway, NJ Open in a separate window Summary of antibody dilutions and conditions used in the immunoblotting analysis of mitochondrial structural and synaptic proteins in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ SHSY5Y cells, mutant APP Swe/ i nd cells and co-immunoprecipitation analysis. ..

Article Title: Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities
Article Snippet: .. To quantify the immunoreactivity of proteins of interest, for each treatment 10–15 photographs were taken at ×20 magnification. table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody – species and dilution Purchased from Company, State Secondary antibody, dilution, Alexa fluor dye Purchased from Company, City & State Drp1 Rabbit Polyclonal 1:300 Novus Biological,Littleton, CO Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe,Grand Island, NY SYN Rabbit Polyclonal 1:300 Protein Tech Group, Inc, Chicago, IL Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY PSD95 Rabbit Monoclonal 1:300 Abcam,Cambridge, MA Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa594 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ Double labeling immunofluorescence analysis of Drp1 and A β To determine the interaction between Drp1 and Aβ, we conducted double-labeling immunofluorescence analysis, using an anti-Drp1antibody (rabbit polyclonal, Santa Cruz Biotechnology) and 6E10 (Covance). ..

Article Title: Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities
Article Snippet: .. To quantify the immunoreactivity of proteins of interest, for each treatment 10–15 photographs were taken at ×20 magnification. table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody – species and dilution Purchased from Company, State Secondary antibody, dilution, Alexa fluor dye Purchased from Company, City & State Drp1 Rabbit Polyclonal 1:300 Novus Biological,Littleton, CO Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe,Grand Island, NY SYN Rabbit Polyclonal 1:300 Protein Tech Group, Inc, Chicago, IL Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY PSD95 Rabbit Monoclonal 1:300 Abcam,Cambridge, MA Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa594 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ .. To determine the interaction between Drp1 and Aβ, we conducted double-labeling immunofluorescence analysis, using an anti-Drp1antibody (rabbit polyclonal, Santa Cruz Biotechnology) and 6E10 (Covance).

Immunofluorescence:

Article Title: Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities
Article Snippet: .. To quantify the immunoreactivity of proteins of interest, for each treatment 10–15 photographs were taken at ×20 magnification. table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody – species and dilution Purchased from Company, State Secondary antibody, dilution, Alexa fluor dye Purchased from Company, City & State Drp1 Rabbit Polyclonal 1:300 Novus Biological,Littleton, CO Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe,Grand Island, NY SYN Rabbit Polyclonal 1:300 Protein Tech Group, Inc, Chicago, IL Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY PSD95 Rabbit Monoclonal 1:300 Abcam,Cambridge, MA Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa594 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ Double labeling immunofluorescence analysis of Drp1 and A β To determine the interaction between Drp1 and Aβ, we conducted double-labeling immunofluorescence analysis, using an anti-Drp1antibody (rabbit polyclonal, Santa Cruz Biotechnology) and 6E10 (Covance). ..

Western Blot:

Mutagenesis:

Immunohistochemistry:

Article Title: Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities
Article Snippet: .. To quantify the immunoreactivity of proteins of interest, for each treatment 10–15 photographs were taken at ×20 magnification. table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody – species and dilution Purchased from Company, State Secondary antibody, dilution, Alexa fluor dye Purchased from Company, City & State Drp1 Rabbit Polyclonal 1:300 Novus Biological,Littleton, CO Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe,Grand Island, NY SYN Rabbit Polyclonal 1:300 Protein Tech Group, Inc, Chicago, IL Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY PSD95 Rabbit Monoclonal 1:300 Abcam,Cambridge, MA Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa594 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ Double labeling immunofluorescence analysis of Drp1 and A β To determine the interaction between Drp1 and Aβ, we conducted double-labeling immunofluorescence analysis, using an anti-Drp1antibody (rabbit polyclonal, Santa Cruz Biotechnology) and 6E10 (Covance). ..

Article Title: Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities
Article Snippet: .. To quantify the immunoreactivity of proteins of interest, for each treatment 10–15 photographs were taken at ×20 magnification. table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody – species and dilution Purchased from Company, State Secondary antibody, dilution, Alexa fluor dye Purchased from Company, City & State Drp1 Rabbit Polyclonal 1:300 Novus Biological,Littleton, CO Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe,Grand Island, NY SYN Rabbit Polyclonal 1:300 Protein Tech Group, Inc, Chicago, IL Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY PSD95 Rabbit Monoclonal 1:300 Abcam,Cambridge, MA Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa594 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ .. To determine the interaction between Drp1 and Aβ, we conducted double-labeling immunofluorescence analysis, using an anti-Drp1antibody (rabbit polyclonal, Santa Cruz Biotechnology) and 6E10 (Covance).

Labeling:

Article Title: Aqua-soluble DDQ reduces the levels of Drp1 and A β and inhibits abnormal interactions between A β and Drp1 and protects Alzheimer’s disease neurons from A β - and Drp1-induced mitochondrial and synaptic toxicities
Article Snippet: .. To quantify the immunoreactivity of proteins of interest, for each treatment 10–15 photographs were taken at ×20 magnification. table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody – species and dilution Purchased from Company, State Secondary antibody, dilution, Alexa fluor dye Purchased from Company, City & State Drp1 Rabbit Polyclonal 1:300 Novus Biological,Littleton, CO Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe,Grand Island, NY SYN Rabbit Polyclonal 1:300 Protein Tech Group, Inc, Chicago, IL Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa488 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY PSD95 Rabbit Monoclonal 1:300 Abcam,Cambridge, MA Goat anti-rabbitBiotin 1:400, HRP-Streptavidin(1: 200),TSA-Alexa594 KPL, Gaithersburg, MDVECTOR Laboratories INC, Burlingame, CAMolecular Probe, Grand Island, NY Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of Drp1, Synaptophysin and PSD95 in the SHSY5Y cell treated with DDQ, Aβ, Aβ+DDQ and DDQ + Aβ Double labeling immunofluorescence analysis of Drp1 and A β To determine the interaction between Drp1 and Aβ, we conducted double-labeling immunofluorescence analysis, using an anti-Drp1antibody (rabbit polyclonal, Santa Cruz Biotechnology) and 6E10 (Covance). ..

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